microbiomes 2021 46 Search Results


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Image Search Results


Summary of included studies.

Journal: International Journal of Surgery (London, England)

Article Title: The microbial composition of pancreatic ductal adenocarcinoma: a systematic review of 16S rRNA gene sequencing

doi: 10.1097/JS9.0000000000001762

Figure Lengend Snippet: Summary of included studies.

Article Snippet: Comparisons of oral, intestinal, and pancreatic bacterial microbiomes in patients with pancreatic cancer and other gastrointestinal diseases. Chung, M et al . 2021. USA , PDAC ( n =24) Ampullary adenocarcinoma ( n =8) Cholangiocarcinoma ( n =4) Benign controls ( n =16) 316 oral samples (52 tongue swab, 46 buccal swab, 35 supragingival swab, 48 saliva samples) 6 normal pancreatic tissues 33 pancreatic tumour samples 22 Duodenum tissue 34 jejunum swab, 19 bile duct swab samples, 21 pancreatic ducts, , 16S rRNA gene analysis performed V3-V4 hypervariable regions of the 16SrRNA gene. Sequence quality checking and denoising were performed using the DADA2 Illumina sequence denoising process. Taxonomic classification, alignment, and phylogenetic tree building were completed using QIIME2. , Bacterial communities from tongue and saliva samples clustered together, while those from buccal and supragingival samples formed another cluster. The saliva bacteria samples were: Capnocytophaga gingivali Fusobacterium nucleatum Streptococcus ASVs. Matched bacteria present between saliva and any pancreatic tissue were: Fusobacterium Rothia Saccharibacteria Oribacterium Streptococcus , Not mentioned , Findings indicate that oral, intestinal, and pancreatic bacterial microbiomes overlap but exhibit distinct co-abundance patterns in patients with pancreatic cancer and other gastrointestinal diseases. , Yes, available. PRJNA558364..

Techniques: Sequencing, Positive Control, Clinical Proteomics, Bacteria, Control, Biomarker Discovery, Generated, Extraction, Transmission Assay, Software, Amplification, Formalin-fixed Paraffin-Embedded, Diagnostic Assay, Marker, Construct, Transplantation Assay